Aldo Emanuel Pinto

Stellixir Biotech

• Testing of a novel drug on Osteosarcoma cell lines.
• Cell culture basics, maintenance of cell lines.
• Cell Apoptosis assays, MTT assay, Annexing V Flow cytometry and Cryopresevation.

Ramaiah University of Applied Sciences

• Computationally identified and annotated 5 protein families from transcriptomic data extracted from Psammophis Schokari.
• Identified valid transcriptomic sequences from transcriptomic data using sequence allignment tools.
• Identified proteins encoded by the RNA sequences, annotated the presences of important domains, such as the binding domains, motifs corresponding to protein activity and disordered regions.
• Identified relevant homologs within and across species and analysed conserved regions between homologs.
• Identified cysteine rich regions to aid in building of the 3D protein structure.
• Computationally built a 3D protein using Phyre3D as well as Alphafold by deepmind google.
• Deduced a possible combination of snake protein families making up the composition of the snake venom from Psammophis Schokari

University of Turin

• Pharmacological stimulation of PIEZO1 channel in Human blood brain barrier endothelial cell mono culture (hCMEC/D3).
• Cell culture of hCMEC/D3 cells in a monolayer, silencing of hCMEC/D3 cells in culture
• Electrophysiological characterization of PIEZO1 channel induced currents, response to stimuli, density of net currents and membrane potential recordings using Patch-clamping experiments.
• Subsequent statistical analysis of recorded data to quantify the significance using computational tools.

B.Sc(Honors) · Biotechnology

M.Sc · Cellular and Molecular Biology